Abstract: In order to further provide a theoretical basis for groundwater utilization and safe mining in the Shenfu mining area. In this paper, based on the analysis of hydrogeological structure, the hydraulic connection between the Salausu Formation aquifer, the weathered bedrock aquifer of the Zhiluo Formation and the bedrock aquifer of the Zhiluo Formation in the Shenfu mining area is analyzed using a combination of hydrochemistry, multivariate statistics and ²H, ¹⁸O and ³H isotope methods. The results show that the pH of all aquifers in the study area is between 7 and 11, which is alkaline water. The average TDS values of groundwater from the Salawusu Formation and the weathered bedrock of the Zhiluo Formation are 294.55 mg/L and 267.72 mg/L, respectively, which are freshwater with low mineralization. The average value of TDS in bedrock groundwater of the Zhiluo Formation is 867.35 mg/L, with a high degree of mineralization. The main hydrochemical types of the Salawusu Formation aquifer and the weathered bedrock aquifer of the Zhiluo Formation are both HCO₃−Ca type water, and the main hydrochemical types of the bedrock aquifer of the Zhiluo Formation are HCO₃−Na type water and HCO₃·SO₄−Na type water. The results of the clustering analysis of water chemistry indicate that the Salawusu Formation aquifer has a certain degree of correlation with the weathered bedrock aquifer of the Zhiluo Formation. The hydrogen and oxygen isotope signatures show that the groundwater recharge sources of the weathered bedrock of the Salawusu Formation and the Zhiluo Formation are the same, and the groundwater recharge sources of the bedrock of the Zhiluo Formation are different from both. The age of groundwater was calculated based on the tritium content in groundwater of each aquifer, and it was concluded that the age of groundwater of the Salawusu Formation is small and the age of groundwater of the bedrock of the Zhiluo Formation is relatively old. Comprehensive water chemistry and environmental isotope information show that the hydraulic connection between the Salawusu Formation aquifer and the weathered bedrock aquifer of the Zhiluo Formation is close, and the hydraulic connection between the two aquifers mentioned above and the bedrock aquifer of the Zhiluo Formation is poor. The research results will provide scientific basis for coal mining, rational use of groundwater and water damage prevention and control in the study area.