Abstract: The groundwater in Ankang Basin is rich in strontium, but its hydrochemical characteristics and the mechanisms of strontium enrichment remain unclear. This study employed a combination of hydrochemical analytical methods (including correlation analysis, saturation index (SI), and major ion ratios) and isotopic analysis to systematically investigate the characteristics and genetic mechanisms of strontium enrichment in the study area. The results showed that the groundwater was strontium-rich and weakly alkaline with low-salinity overall. The primary hydrochemical type of the groundwater was HCO₃-Ca. Groundwater mainly came from atmospheric precipitation and was less affected by evaporation. The hydrochemistry of the groundwater was mainly controlled by the weathering of carbonate rocks. The main source of strontium enrichment in groundwater was attributed to the weathering and dissolution of secondary carbonates (mainly calcite) in Quaternary loose sediments and limestone. Although there was a certain degree of alternating cation adsorption, but the exchange intensity was limited, which had little effect on the enrichment of strontium in groundwater. Human activities, such as agricultural practices and domestic wastewater, promoted the enrichment of strontium in the groundwater to some extent. These findings can provide geological support for the scientific development, management, and utilization of groundwater resources in the Ankang area.