Abstract: This paper measured the homogenization temperature, salinity and S-H-O isotopic compositions of the quartz inclusions in order to study the geochemical characteristics and genesis of the Yinchang gold deposit. The results show that the ore-forming source is mainly from the epimetamorphic clastic rocks of Triassic Zagunao Group. The metallogenic fluid is a mixture of juvenile fluid with metamorphic water. The metallogenic temperature is low or medium. The genetic type of Yinchang gold deposit is a low-or-medium temperature hydrothermal deposit controlled by fracture.