Abstract: Occurred in Zhenzhumen Formation of Proterozoic Laoling Group, the Huanggoushan Pb-Zn deposit is controlled by stratum and ductile shear belt, serving as one of the most representative deposits in Laoling polymetallic metallogenic belt, southernJilinProvince.Hydrothermal superimposed mineralization was occured in the late ore-forming stage and exerted an important influence on further enrichment of ore-forming elements.In order to explore the sources and characteristics of the fluid, the microthermometry and C-D-O isotopic geochemistry methods of fluid inclusions are used to research the nature of the ore-forming fluids in each stage of hydrothermal superimposed mineralization. Results indicate thatthe ore-forming fluidof the first stage belongs to mid-low temperature, low-salt NaCl-H₂O system. The δDH₂O, δ¹⁸OH₂O and δ¹³C_V-PDB of these fluid inclusions are -74.8‰~-87.4‰, 8.3‰~9.8‰ and -9.6‰~-10.8‰, respectively, showingsome characteristics of magma water. The ore-forming fluid of the second stage belongs to low-temperature, low-salt NaCl-H₂O system. The δDH₂O, δ¹⁸OH₂O and δ¹³C_V-PDB of these fluid inclusions are -91.4‰~-93.9‰, 3.3‰~4.7‰ and -9.5‰~-10.5, respectively, which show some characteristics about mixture fluid of meteoric water and magma water, however the magma water still play a main role.