Abstract: We are facing new challenges in the management of natural resources in the construction of ecological civilization. As the first layer of the natural resources, the ground substrate layer is the basis that supports and nurtures the ground cover layer. Therefore, it is a realistic and urgent task to conduct ground substrate layer survey of natural resources.Langfang Natural Resources Comprehensive Survey Center of China Geological Survey took the lead in carrying out a pilot survey of the ground substrate layer of natural resources in Baoding city.this paper summarized the element-index system for the ground substrate layer survey, and determined the bottom boundaries for the survey in different regions and of different types. For the first time, a technical route of "indoor research, field survey, database construction simulation, platform service" and the methods of "data modification, remote sensing interpretation, comprehensive mapping, profile measurement, geophysical survey, geochemical survey, and engineering Construction (drilling, trenching, well exploration, etc), sampling and testing, comprehensive research" were established and applied. The authors aim to provide reference for the investigation of the ground substrate layer survey of natural resources.